bluescript sk ii (1) vector Search Results


biotinylated maackia amurensis lectin ii  (Vector Laboratories)
95
Vector Laboratories biotinylated maackia amurensis lectin ii
Biotinylated Maackia Amurensis Lectin Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated maackia amurensis lectin ii/product/Vector Laboratories
Average 95 stars, based on 1 article reviews
biotinylated maackia amurensis lectin ii - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier
pmal vector  (New England Biolabs)
96
New England Biolabs pmal vector
Pmal Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmal vector/product/New England Biolabs
Average 96 stars, based on 1 article reviews
pmal vector - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
pgl2 basic vector  (OriGene)
93
OriGene pgl2 basic vector
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Pgl2 Basic Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgl2 basic vector/product/OriGene
Average 93 stars, based on 1 article reviews
pgl2 basic vector - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
biotin conjugated maackia amurensis lectin ii  (Vector Laboratories)
94
Vector Laboratories biotin conjugated maackia amurensis lectin ii
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Biotin Conjugated Maackia Amurensis Lectin Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin conjugated maackia amurensis lectin ii/product/Vector Laboratories
Average 94 stars, based on 1 article reviews
biotin conjugated maackia amurensis lectin ii - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
alexa 488 conjugated griffonia simplicifolia lectin ii  (Vector Laboratories)
96
Vector Laboratories alexa 488 conjugated griffonia simplicifolia lectin ii
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Alexa 488 Conjugated Griffonia Simplicifolia Lectin Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa 488 conjugated griffonia simplicifolia lectin ii/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
alexa 488 conjugated griffonia simplicifolia lectin ii - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
exnasetm ii  (Clonexpress inc)
90
Clonexpress inc exnasetm ii
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Exnasetm Ii, supplied by Clonexpress inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/exnasetm ii/product/Clonexpress inc
Average 90 stars, based on 1 article reviews
exnasetm ii - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
biotinylated gs ii  (Vector Laboratories)
93
Vector Laboratories biotinylated gs ii
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Biotinylated Gs Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated gs ii/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
biotinylated gs ii - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
mal ii  (Vector Laboratories)
99
Vector Laboratories mal ii
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Mal Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mal ii/product/Vector Laboratories
Average 99 stars, based on 1 article reviews
mal ii - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
puc19  (New England Biolabs)
96
New England Biolabs puc19
(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or <t>PGL2</t> basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.
Puc19, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/puc19/product/New England Biolabs
Average 96 stars, based on 1 article reviews
puc19 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier

Image Search Results


(A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or PGL2 basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.

Journal: Biochimica et biophysica acta. Gene regulatory mechanisms

Article Title: Interaction of Positive Coactivator 4 with Histone 3.3 Protein is Essential for Transcriptional Activation of the Luteinizing Hormone Receptor Gene

doi: 10.1016/j.bbagrm.2018.09.002

Figure Lengend Snippet: (A, above) Western blot showing PC4, H3 and acetylated H3 levels in MCF7 cells transfected with scrambled or PC4 siRNA followed by treatment with or without TSA (500ng/ml) for 16 h revealed by PC4, H3 and specific H3 acetylated antibodies to H3 Lys residues (K9, K14, K18, K23, K27 and K36). (A, below) Western blot showing PC4 and H3.3 levels in MCF7 cells treated with scrambled or PC4 siRNA followed by treatment with or without TSA. β-actin is shown as a loading control. Results are representative of three independent experiments. (B) Effect of endogenous PC4 knock-down by siRNA on LHR promoter activity. Cells transfected with scrambled (control) or PC4 siRNA followed by transfection of LHR promoter construct or PGL2 basic vector and later treated with or without TSA prior to measurement of promoter activity. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.001). (C) ChIP analysis illustrates relative enrichment of H3K9-Ac onto the LHR promoter of cells transfected with scrambled or PC4 siRNA cultured in the presence or absence of TSA. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). (D) H3.3 enrichment at the LHR promoter assessed in cells treated with and without TSA following PC4 knockdown. Different superscript letters indicate significant differences (Dunn’s multiple comparison test, P < 0.05). Representative western blot showing knock-down of PC4 protein in MCF7 cells following PC4 siRNA treatment. Data represent mean ± SE of two independent experiments performed in triplicates.

Article Snippet: Expression vectors and cell culture The reporter gene construct containing the LHR promoter was generated by cloning the human LHR gene promoter region (−176 to +1) into the SacI/BglII sites of the pGL2 basic vector [ 12 ]. pCMV6-PC4 was purchased from Origene (Rockville, MD) and used as PCR template for generation of constructs expressing PC4-Flag protein in MCF7 cells. p3XFLAG-PC4 vector was created by inserting PCR-amplified PC4 cDNA into the EcoRI and KpnI sites of the p3XFLAG-CMV-7.1 vector (Sigma) [ 11 ].

Techniques: Western Blot, Transfection, Activity Assay, Construct, Plasmid Preparation, Cell Culture